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Osteoblast progenitors derived from adult bone-marrow (MDP-osteoblast) are now available as a model for evaluating growth factor (GF) involvement in bone formation. The MDP-osteoblast secreted substantial amounts of bone-GFs during periods of in vitro culture. On the other hand, when these cells were injected into the mouse subcutaneous tissue, no osteogenic effect was detected. The present study was undertaken to define the biological activity of these GFs, as well as to determine whether there was synergism between the GFs. The results show that the MDP-osteoblast responds to the GFs produced by the MDP-osteoblasts. Indeed, the GFs promoted the proliferation of the MDP-osteoblast. However, the MDP-osteoblast was not capable of forming bone in vivo when transplanted subcutaneously in non-osteopenic mice. Thus, the GFs from the MDP-osteoblast stimulate the growth of the MDP-osteoblast, but do not induce osteogenesis.Evaluation of hepatic reserve in patients with liver cirrhosis by multidetector spiral computed tomography.
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